A Study of Enzymes

First published in 1990, this comprehensive monograph consists of two parts: Volume I, entitled Enzyme Catalysis, Kinetics, and Substrate Binding; and Volume II, entitled Mechanism of Enzyme Action. This particular volume provides important information for both the novice and the seasoned investigator.

Enzyme Catalysis and Steady-State Kinetics: Historical Introduction, Theories of Enzyme Catalysis, and Some Elementary Considerations of Enzyme Kinetics. A Description of Steady-State Kinetics and Quasi- or Rapid Equilibrium Kinetics by a Development of the Rate Expressions for Several Selected Mechanisms and Their Characteristics. Inhibition and Product Inhibition. Effects of pH and Temperature. Effect of Metal Cofactors on the Reaction Velocity. Enzyme Kinetics and Substrate Binding: Some Remarks on Isotope Exchange Studies and Kinetic Isotope Effects. Non-Michaelis-Menten Kinetics and Allosteric Kinetics. Equilibrium Ligand Binding-Multiple Equilibria. Some Complex Kinetic Mechanisms and Treatment of Enzyme Kinetic Data. Kinetics of the Transient Phase or Pre-Steady-State Phase of Enzyme Reactions. Appendix 1. Appendix 2. References. Index.