Vertebrate Embryogenesis

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.  
Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments

            Douglas W. Houston

 

2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos

            Thao Nguyen, Timothy J. Mitchison, and Martin Wühr

 

3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos

            Michael D. Sheets

 

4. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

            Alexander A. Tokmakov and Ken-Ichi Sato

 

5. Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis

            Ken-ichi Sato and Alexander A. Tokmakov

 

6. Embryological and Genetic Manipulation of Chick Development

            Laura S. Gammill, Bridget Jacques-Fricke, and Julaine Roffers-Agarwal

 

7. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos

            Ana Paula Azambuja and Marcos Simoes-Costa

 

8. Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens

            Triveni Menon and Sreelaja Nair

 

9. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)

            Karoline Holler and Jan Philipp Junker

 

10. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos

            Clare E. Buckley

 

11. Ex Utero Culture and Imaging of Mouse Embryos

            Sonja Nowotschin, Vidur Garg, Anna Piliszek, and Anna-Katerina Hadjantonakis

 

12. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

            Edwina McGlinn, Miriam Holzman, and Jennifer H. Mansfield

 

13. Reptile Embryology and Regeneration

            Matthew K. Vickaryous and Emily A.B. Gilbert

 

14. Manipulation of Developmental Function in Turtles with Notes on Alligators

            Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, and Judith A. Cebra-Thomas

 

15. Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes

            Amanda Butler, Dawn Owens, Mary Lou King, and Tristan Aguero

 

16. Visualizing the Balbiani Body in Zebrafish Oocytes

            KathyAnn L. Lee and Florence L. Marlow

 

17. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis

            Allison Jamieson-Lucy and Mary C. Mullins

 

18. Generation of Xenopus Haploid, Triploid, and Hybrid Embryos

            Romain Gibeaux and Rebecca Heald

 

19. Germ Cell Transplantation in Avian Species

            Young Hyun Park, Young Min Kim, and Jae Yong Han

 

20. Production of Germ-Line Chimeras in Zebrafish

            Taiju Saito, Rie Goto, Nicola Rivers, and Etsuro Yamaha

 

21. A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring

            Zsolt Csenki and Ferenc Mueller

 

22. Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT)

            Sukumal Prukudom, Kannika Siripattarapravat, William Poulos, and Jose B. Cibelli

 

23. An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish

            Cara E. Moravec and Francisco J. Pelegri

 

24. Modeling Embryonic Cleavage Patterns

            Dmitry Ershov and Nicolas Minc

 

25. Laboratory Guidelines for Animal Care

            Marcelo Couto and Charles Cates