Genetic Variance Detection

Chapter 1 Basics of automated, high-accuracy mutation screening with the WAVE[registered] Nucleic Acid Fragment Analysis Systems; Chapter 2 Influence of PCR on DHPLC Polymorphism Characterization; Chapter 3 Denaturing High Performance Liquid Chromatography (DHPLC), an important mutation detection technique in mutation analysis of the breast and ovarian cancer predisposing genes BRCA1 and BRCA2; Chapter 4 Mutation detection in the tuberous sclerosis genes TSC1 and TSC2 by DHPLC; Chapter 5 Rapid analysis to detect base changes in human mitochondrialDNA by restriction enzyme analysis followed by denaturing high performance liquid chromatography; Chapter 6 Mutation analysis of LDLR mutations in patients with familial hypercholesterolaemia; Chapter 7 Novel DHPLC based strategy for identification and characterization of (bi)-clonal T-cell receptor targets for the detection of minimal residual disease in childhood acute lymphoblastic leukemia; Chapter 8 SIRPH: a rapid, quantitative SNuPE-IP RP HPLC method for simultaneous methylation analysis at multiple CpG sites; Chapter 9 Isolation and sequencing of heteroduplex fragments from DHPLC analysis of somatic, low-f requency mutations; Chapter 10 Analysis of promoter methylation using denaturing high-performance liquid chromatography (DHPLC)