Practical Fluorescence Spectroscopy

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy.

Chapter 1. Theory of Light and Light Interaction with Matter Basics of Light Light Polarization Interaction of Light and Chromophores Interactions Among Chromphores Scattering Summary Chapter 2. Experimental Basics Types of Cuvettes Types of Filters Measuring Transmittance/Absorbance Factors Affecting the Precision of Absorption Measurement Effect of Sample Scattering on Measured Transmission and Abosrbance Measuring Emission Efficiency of Collecting Emission: Measuring Quantum Yield Wavelength Calibration Instrucment Response Lamp Profile Peaks and Spectral Shapes Standard Deviation and Height Sums of Peaks Noise-Signal-to-Noise Ratio Sampling Rate Bandwidth Artifacts and Errors Spectral Overlap Peak Normalization Residuals Interpolation Chapter 3. Experiments Introduction Experiments Chapter 4. Fluorescence-Steady-State Phenomena Introduction Experiments Chapter 5. Steady-State Fluorescence: Applications Introduction Experiments Chapter 6. Steady-State Fluorescence Polarization: Anisotropy Introduction How to Select and Check Polarizers Transition Moments Polarized Absorption Emission Polarization (Anisotropy) Fundamental and Limiting Anistropies Consequences of Linear Transition Moments Determining the G-Factor for Spectrofluorometer Experiments Chapter 7. Fluorescence: Time-Resolved Phenomena Introduction Experiments Chapter 8. Advanced Experiments Introduction Experiments