New & Emerging Proteomic Techniques

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

On-Chip Protein Synthesis for Making Microarrays Niroshan Ramachandran, Eugenie Hainsworth, Gokhan Demirkan, and Joshua LaBaer RCA-Enhanced Protein Detection Arrays Brian B. Haab and Paul M. Lizardi Antibody Microarrays Using Resonance Light-Scattering Particles for Detection Bernhard H. Geierstanger, Petri Saviranta, and Achim Brinker Chemical Proteomics Profiling of Proteasome Activity Martijn Verdoes, Celia R. Berkers, Bogdan I. Florea, Paul F. van Swieten, Herman S. Overkleeft, and Huib Ovaa Two-Dimensional Difference Gel Electrophoresis Terence L. Wu Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors Ewa Folta-Stogniew Surface Plasmon Resonance Imaging Measurements of Protein Interactions With Biopolymer Microarrays Terry T. Goodrich, Alastair W. Wark, Robert M. Corn, and Hye Jin Lee Surface Plasmon Resonance Mass Spectrometry for Protein Analysis Dobrin Nedelkov and Randall W. Nelson High-Throughput Affinity Mass Spectrometry Urban A. Kiernan, Dobrin Nedelkov, Eric E. Niederkofler, Kemmons A. Tubbs, and Randall W. Nelson Isotope-Coded Affinity Tags for Protein Quantification Christopher M. Colangelo and Kenneth R. Williams Proteomic Analysis by Multidimensional Protein Identification Technology Laurence Florens and Michael P. Washburn Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites Hui Zhang and Ruedi Aebersold N-Glycosylation Analysis Using the StrOligo Algorithm Martin Ethier, Daniel Figeys, and Hélene Perreault MALDI-MS Data Analysis for Disease Biomarker Discovery Weichuan Yu, Baolin Wu, Junfeng Liu, Xiaoye Li, Kathy Stone, Kenneth R. Williams, and Hongyu Zhao Using the Global Proteome Machine for Protein Identification Ronald C.Beavis Index