Epidermal Growth Factor Methods & Protocols

A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

A Historical Perspective of the EGF Receptor and Related Systems Francis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics, and Tarun B. Patel Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, and Paul J. Bertics Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay Jacqueline M. Lafky, Andre T. Baron, and Nita J. Maihle Analysis of EGF Receptor Interactions With the a Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs Deepti Chaturvedi, Francis Edwin, Hui Sun, and Tarun B. Patel Regulator of Epidermal Growth Factor Signaling: Sprouty Esther Sook Miin Wong and Graeme R. Guy Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway Oliver M. Fischer, Stefan Hart, and Axel Ullrich A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, and Carl P. Blobel In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases Menachem Katz, Yaron Mosesson, and Yosef Yarden Assays to Monitor Degradation of the EGF Receptor Mirko H. H. Schmidt and Ivan Dikic Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members Adriano Marchese Epithelial Cell Migration in Response to Epidermal Growth Factor Reema Zeineldin and Laurie G. Hudson Motility Signaled From the EGF Receptor and Related Systems Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffith, and Douglas A. Lauffenburger Methods for Determining the Proliferation of Cells in Response to EGFR Ligands Gregory J. Wiepz, Francis Edwin, Tarun B. Patel, and Paul J. Bertics Clinical Advancement of EGFR Inhibitors in Cancer Therapy Prakash Chinnaiyan and Paul M. Harari Index