Methods in Enzymology v399

Covers chemical biology, ubiquitin derivatives and ubiquitin-like proteins, deubiquitinating enzymes, proteomics as well as techniques to monitor protein degradation. This work includes chapters that focus on application of techniques. It covers topics such as: E1 Enzymes, E2 Enzymes, E3 Enzymes, Proteasomes, and Isopeptidases.

SECTION I. UBIQUITIN AND UBIQUITIN DERIVATIVES SECTION II. UBIQUITIN-BINDING DOMAINS SECTION III. METHODS TO STUDY THE PROTEASOME SECTION IV. IDENTIFICATION AND CHARACTERIZATION OF SUBSTRATES AND UBIQUITIN LIGASES SECTION V. GENOME- AND PROTEOME-WIDE APPROACHES TO IDENTIFY SUBSTRATES AND ENZYMES SECTION VI. REAL TIME/NON-INVASIVE TECHNOLOGIES SECTION VII. SMALL MOLECULE INHIBITORS SECTION VIII. GENERALLY APPLICABLE TECHNOLOGIES